TREATMENT OF INFECTIOUS PATHOLOGY AND RESTORATION OF NORMAL HUMAN UROGENITAL FLORA BY MEANS OF HIGH-SELECTIVITY ELECTROMAGNETIC RADIATION IN COMBINATION WITH PROBIOTICS

S.I. Petrenko, *I.V. Timofeyev, *N.G. Perminova

Research and Production Enterprise “Bionix”, N. Novgorod; *State Research Center of Virology and Biotechnology “Vector”, 630559, Koltsovo, Novosibirsk region, Russia

e-mail: bionix@sandy.ru

The contemporary onrushing development of information technologies has resulted in advent of new approaches in modern medicine and biology – informational physiotherapeutical methods of disease prevention and treatment. A considerable number of studies deal with action of infra-red (IR) electromagnetic radiation of non-thermal intensity on biological objects. The world’s first highly effective antimicrobial device of physical therapy, invented in Russia on the basis of Russian patent “The device for influencing the activity of a biological cell” [1] is a considerable step forward in treatment of some infectious diseases.

The appliance is designed for restoration of urogenital microflora during clinical and out-patient treatment of prostatitis, urethritis, cervicitis, orchoepididymitis, salpingo-oophoritis with chlamidyal, mycoplasmatic, ureoplasmatic, staphylococcal, trichomonadal, candidal and a number of other etiologies. The device can be employed for monotherapy and in combination with standard physiotherapy methods and probiotics. “Uro-Biophone” is built around several microprocessors and is an automatic self-testing device. The appliance has passed a complete cycle of technical and clinical tests. Certified by the Ministry of Health of the Russian Federation, it has been in full-scale production since 1998.

When operated the device emits modulated electromagnetic waves of 0.7-0.8mkm band (99% emissive power) and polarized electromagnetic waves of 3-10000 mkm (below 0.1% emissive power), whose spectrum is identical to that of the inactivated pathogenic strains given below: Chlamydiaceae, Spirochaetaceae (Spirochaeia, Treponema, Cristispira, Borrelia), Neisseriaceae (Neisseria, Moraxella, Acinetobacter, Kingella), Micrococcaceae (Micrococcus, Staphylococcus, Stomatococcus, Planococcus), Enterobacteriaceae (Escherichia, Klebsiella, Morganella, Salmonella, Shigella, Yersinia, Proteus etc.), Trichomonas, Pseudomonadaceae, Mycobacteriaceae, Gardnerella, Mobiluncus, Bacteroidaceae, Enterococcus, Mycoplasmataceae (Mycoplasma, Ureaplasma), Candida, Papovaviridae.

The polarized IR-radiation inactivates pathogenic microorganisms causing conformational changes in the specific proteins of the plasmatic membrane [2,3] which results in appearance of a cut-off potential E_off on the plasmatic membrane of the pathogen, leading to decrease in the intensity of biochemical processes in the microbial cell and increase of its vulnerability. Complete purge of the macroorganism of the above pathogens is achieved due to their active replacement by commensal microflora and intensive elimination of pathogenic strains by the elements of the immune system.

An illustrative example is furnished in diagram (Fig. 1) which demonstrates dependence of the Staphylococcus aureus strain activity in the patient with slow inflammatory process diagnosed as non-specific urethritis and the culture-forming units (CFU/ml) dynamics in the urethral culture after the IR-therapy by means of “Bio-Phone”. The plasmatic membrane potential was measured by a field remote method through analysis of the physical fields formed by a monoculture colony. The patient received treatment consisting of 4 daily sessions during 3 days. As evident from the diagram complete elimination of Staphylococcus aureus occurred in the patient’s urethra after 50 hours from the start of the physical therapy.

Duration of an IR-therapy session remains constant and is 24 seconds. Frequency of administering sessions is determined by the attending physician in accordance with the character of the inflammatory condition and may vary from one session in 2-3 days to 48 sessions administered daily (average frequency being 3-6 sessions a day). The treatment schedule should be planned so that the pathogenic strains are kept inactive throughout the entire treatment period. With diligent observance of the recommended therapy method complete purge of the organism of the above-mentioned pathogenic agents occurs in 98% cases. For patients with a pronounced immune deficiency duration of treatment may be increased tenfold.

However, elimination of one or several pathogenic strains may initially make the organism somewhat less resistant to recurrent colonization by the same kind of strains since the colonization resistance of commensal microorganisms, which counteract colonization by pathogens, stays low and their variety is limited.

To enhance resistance of human organism to recurrent colonization by pathogenic flora, say, staphylococci, it is necessary to restore commensal flora of the same kind. To achieve it, the macroorganism must first be purged of pathogenic microorganisms inhibiting the activity of commensal staphylococci. So, when operated “Uro-Biophone” inactivates not only the pathogenic staphylococcal strains and their toxins but also other pathogenic strains of bacteria, viruses, fungi, prions, monads (and their toxins) inhibiting commensal staphylococci, thus restoring the activity of the latter and favoring restoration of the commensal staphylococci strains quantity in the course of time.

The level of colonization of the organism by strains of microorganisms may be expressed by value S which is determined by formula:

where N is a number of types of commensal strains present in the given organism; M is maximal number of types of commensal strains of a certain genus which may colonize a human organism.

A generalized restoration process for commensal microflora of genus Staphylococcus with various commensal staphylococci colonization levels in three different patients is shown on fig. 2 with colonization parameters S_st = 10%, 50% and 90%.

The time required for restoration of commensal flora may vary over a wide range, from one month to 2 years, depending on the state of the commensal flora of a particular genus in the human organism, on the patient’s age, performance of his system, to what degree the commensal probiotic flora dominates over the pathogenic one in the food (especially in meat and dairy products), on environmental factors and social surroundings of the patient.

Fig.3 presents the schematic diagrams illustrating operation of “Uro-Biophone” in the perspective of changes taking place both in the activity and quantity of types of the commensal and pathogenic strains of a certain genus by stages during treatment. For the purposes of illustration the radiation spectrum of each strain is shown as an individual spectral line positioned on axis X, while the potential at the plasmatic membrane E (and the respective metabolic activity) is located at Y axis.

It is worthy of note that the transmembrane potential of pathogenic microorganisms E_path is subject to wide variations and may be much grater than that of normal somatical cells, whereas the transmembrane potential of commensal microorganisms E_com cannot exceed the normal transmembrane potential of the cells in mammals

From the curves in Fig.3 it will be obvious that during treatment of patients with urogenital infections by means of “Uro-Biophone” pathogenic strains of some genus are eliminated (N_path ® 0 ), the activity of the commensal strains of the same genus improves (E_com » E_n ), the quantity of the commensal strain types approaches the normal level (N_com » M ) and the colonization ratio grows (S ® 1).

The results of 1865 in vitro experiments where “Uro-Biophone” was used to act on various representatives of genus Mycobacteriaceae, including virulent strains M.H₃₇Rv, M.bovinus-8, M.avium; avirulent strains M.H₃₇Ra and saprophile strains M.smegmatis are presented in the table below.

As evident from the table data the inhibited growth of the virulent laboratory strains is accompanied by 15% increase in growth ratio of avirulent strains M. H₃₇Ra and 166% rise in growth ratio of strains M. smegmatis.

Thus, the mentioned experiments in vitro clearly demonstrate the operating principle of “Uro-Biophone”, its high selectivity and efficacy as compared to other known methods of managing pathogenic microorganisms.

1. S.I. Petrenko – Russian patent ¹2055604 of 13.09.93ã. 6À 61 Ì 37/00, À 61 Í 39/00

2. I.V. Timofeyev et al. IR-band electromagnetic radiation of non-thermal intensity and the “Hormesis” phenomenon in proteins //Vestnik ISRICA ¹7, 2000, pp56-58.

3. M.V. Maslovsky et al. The biological action mechanism of low-intensity electromagnetic fields. //Papers of the Russian Conference: Organism and the environment: life support and human safety in extreme conditions, Moscow, September 26-29, 2000, v.1, pp.272-273.